Aim : To study the digestion of protein
a) MV: Absence of presence of enzyme
b) RV : Condition of solution
c) FV: Temperature//enzyme concentration//volume of albumen suspension
Observations
| Test tube | Condition of content | |
| Beginning of experiment | End of experiment | |
| A | Cloudy | Less cloudy |
| B | Cloudy | Clear |
| C | Cloudy | Cloudy |
Discussion
1. To prepare an acid medium for the action of pepsin.
2. A: Digestion of albumen is very slow because pepsin is not very active in a neutral medium.
B: Digestion of albumen is very fast because pepsin is very active in acidic medium. The cloudy suspension of albumen turns to a clear solution of amino acids.
C: No digestion of albumen as there are no enzymes.
3. Te cloudy suspension remains unchanged because pepsin is inactive in alkaline medium.
Conclusion : The hypothesis is accepted. Pepsin catalyses the hydrolysis of albumen to polypeptides in an acidic medium.
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