Effect of enzyme concentration on the rate of biochemical reaction

Pg 33 - 4.2

Aim : to study the effect of enzyme concentration on the rate of biochemical reaction
Hypothesis : The higher the enzyme concentration, the higher the rate of biochemical reaction until it reaches a maximum rate.
Variables : a) MV - enzyme concentration
b) RV - Time take for breakdown of starch
c) FV - pH//temperature//substrate concentration
Results :

Test tube	A	B	C	D	E	F
Enzyme concentration (%)	16.7	33.3	50.0	66.7	83.3	100.0
Time take for breakdown of starch (min)	8.0	5.0	3.5	2.5	2.0	1.5
Rate of reaction, 1/time (min-1)	0.13	0.20	0.29	0.40	0.50	0.67

Graph :
Discussion :
1. F
2. A
3. The higher the enzyme concentration, the higher the rate of reaction.

Conclusion:
The hypothesis is accepted. The higher the enzyme concentration, the higher the rate of biochemical reaction (breakdown of starch) until it reaches a maximum rate.