Aim :To study the
effects of hypotonic, hypertonic and isotonic solutions to a plant cell
Discussion:
1a) 0.5 M sucrose solution
b) 0.2 M sucrose solution
c) 0.8 M sucrose solution
2. Plasmolysis
3. 0.8 M sucrose solution is hypertonic to the cell sap
of onion cell. Water molecules moves out of the vacuole by osmosis causing the vacuole
to shrink. The plasma membrane detaches from the cell wall. The cell becomes
flaccid.
4. 0.2 M sucrose solution is
hypotonic to the cell sap of onion cell. Water molecules moves into the vacuole by osmosis causing the vacuole to expand
outwards and press both the vacuole and the cell wall. The cell becomes turgid.
5. The cell becomes turgid again due
to deplasmolysis.
Conclusion
Epidermal cell of onion becomes
turgid when immersed in a hypotonic solution. In 0.5M sucrose solution, which is isotonic, there is no change in shape and condition of the
cell. In a hypertonic solution, the cell becomes flaccid due to loss of water
from the vacuole of the cell.
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