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My name is Poon Cheng Moh. I have been teaching biology in SMK(P) Raja Zarina, Port Klang for 26 years. I sincerely hope that this blog on SPM Biology will be useful to both teachers and students.

Sunday, June 19, 2011

Cloned banana plant

Discuss how you can produce a cloned banana plant.
Suggested answer

Small pieces of meristem tissue/explant are removed from a parent banana plant and cut it into small pieces.
Sterilise the pieces of tissues with dilute sodium hypochlorite solution.
Place each sterile tissue onto a culture medium containing nutrients and growth hormone.
The tissue cells are left to divide by mitosis to produce a mass of loosely arranged and undifferentiated cells called callus.
The callus is then stimulated with shoot stimulating hormones to form multiple shoots.
Separate the shoots in nutrient medium with root stimulating hormones to encourage rooting.
Once the roots grow, the plantlets (little plants) are planted in sterile soil to grow into adult plants.

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