Tissue culture

Step 1: Weigh out 2.5 g of nutrient agar and add to 250 ml of distilled water.
Step 2 : Then add hormone into the agar solution. Heat and stir until the agar dissolves.
Step 3 :Pour the hot nutrient agar into several test tubes about 5 cm3 in height.
Allow the agar to cool and solidify in each test tube.
Step 4 :Germinate some mustard seed in a warm lighted place until the cotyledon starts to unfold.
Step 5 : Cut the tips of the seedling just above the shoot apex (meristem).These tissues are called explants.
Step 6 : Put one explant in each test tube.
Step 7 : Cover the tubes with a cling film and place the tubes into a rack by the window.
Step 8 : The tissue cells are left to divide by mitosis to produce a mass of loosely arranged and undifferentiated cells called callus. The callus will then grow into plantlets(little plants) which will grow into adult plants.